By Ron J. Doyle (Eds.)
Volume 310 of Methods in Enzymology is the 1st quantity committed completely to biofilm learn equipment. It offers a latest resource ebook for nearly any form of experimental process concerning biofilms. It comprises bioengineering, molecular, genetic, microscopic, chemical, non-stop tradition, and actual equipment. This quantity will function a place to begin for destiny developments.
The severely acclaimed laboratory average for greater than 40 years, Methods in Enzymology is likely one of the so much hugely revered guides within the box of biochemistry. due to the fact 1955, every one quantity has been eagerly awaited, usually consulted, and praised by means of researchers and reviewers alike. Now with greater than three hundred volumes (all of them nonetheless in print), the sequence comprises a lot fabric nonetheless appropriate today--truly an important e-book for researchers in all fields of existence sciences
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Additional info for Biofilms
7 S. MOiler, D. R. Korber, G. M. Wolfaardt, S. Molin, and D. E. Caldwell, Appl. Environ. Microbiol. 63, 2432 (1997). 8 G. M. Wolfaardt, J. R. Lawrence, R. D. Robarts, S. J. Caldwell, and D. E. Caldwell, Appl. Environ. Microbiol. 60, 434 (1994). ~i~i ,>i~,;iii~I Silicone tubing ~ Cover glass FIG. 1. Schematic presentation of a biofilm flow system. The entire flow system (A) is connected by standard silicone tubing, except for the tubing going into the pump where we use the more sustainable Marprene tubing.
E. Caldwell, D. R. Korber, and J. R. Lawrence, J. Appl. Bact. Suppl. 74, 52S (1993). 6 D. E. Caldwell, D. R. Korber, and J. R. Lawrence, J. MicrobioL Methods 15, 249 (1992). 7 S. MOiler, D. R. Korber, G. M. Wolfaardt, S. Molin, and D. E. Caldwell, Appl. Environ. Microbiol. 63, 2432 (1997). 8 G. M. Wolfaardt, J. R. Lawrence, R. D. Robarts, S. J. Caldwell, and D. E. Caldwell, Appl. Environ. Microbiol. 60, 434 (1994). ~i~i ,>i~,;iii~I Silicone tubing ~ Cover glass FIG. 1. Schematic presentation of a biofilm flow system.
2 mm/sec (flow channel cross section is 4 mm2). The biofilms are grown with the coverslip turned upward to prevent the sedimentation of f o c k s detached from other parts of the biofilm. Optimal microscopic resolution and hydrodynamic considerations within the flow cell set the maximal biofilm thickness at 100-150 /xm. ] Fixation a n d E m b e d d i n g of H y d r a t e d Biofilm S a m p l e s On-line microscopy performed after general staining as indicated briefly yields information about changes in biofilm structure over time.
Biofilms by Ron J. Doyle (Eds.)